sarcoplasmic endoplasmic reticulum calcium atpase serca 1  (Cell Signaling Technology Inc)

Journal: Journal of Biomedical Science

Article Title: Targeting NLRP3 signaling reduces myocarditis-induced arrhythmogenesis and cardiac remodeling

doi: 10.1186/s12929-024-01032-7

Figure Lengend Snippet: Effects of the NLRP3/CaMKII axis on Ca 2+ regulatory proteins in rat RVOTs. Representative Western blots and summary data for sarcoplasmic/endoplasmic reticulum Ca 2+ ATPase 2a, CaMKII phosphorylated at Thr286, RyR2, total phospholamban, RYR phosphorylated at Ser2808, phospholamban phosphorylated at Thr17, protein kinase A, Cav1.2, NLRP3, nuclear factor-κB, and interleukin-1β expression in the RVOT tissues of the control (healthy rats; n = 7), experimental (rats with myocarditis; n = 7), and treatment (MCC950-treated rats with myocarditis; n = 7) groups. Glyceraldehyde-3-phosphate dehydrogenase served as a loading control. CaMKII, Ca2 + /calmodulin-dependent protein kinase II; RYR, ryanodine receptor; RVOT, right ventricle outflow tract

Article Snippet: For the immunofluorescence-based detection of gap junction proteins, all blots were stained with primary antibodies against sarcoplasmic/endoplasmic reticulum Ca 2+ ATPase 2a (SERCA2a), Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) phosphorylated at Thr286 (pCaMKII), RyR2, total phospholamban (Thermo Fisher Scientific, Rockford, IL, USA), phosphorylated RyR at Ser2808, phospholamban phosphorylated at Thr17 (Badrilla, UK), catalytic subunit of protein kinase A (BD BioSciences, USA), Cav1.2, NLRP3, nuclear factor (NF)-κB, IL-1β, and glyceraldehyde-3-phosphate dehydrogenase (MBL, Japan); all secondary antibodies were conjugated with horseradish peroxidase.

Techniques: Western Blot, Expressing

Journal: Acupuncture in Medicine

Article Title: Electroacupuncture alleviates acute myocardial ischemic injury in mice by regulating the β 1 adrenergic receptor and post-receptor protein kinase A signaling pathway

doi: 10.1177/09645284241298716

Figure Lengend Snippet: Effects of electroacupuncture (EA) on the level of norepinephrine (NE), post-receptor protein kinase A (PKA) signaling pathway and expression levels of L-type voltage-gated calcium channel α1 C (Cav1.2), serine phosphorylated 16-phospholamban (p-PLB s16 ) and sarcoplasmic reticulum (SR) Ca 2+ -adenosine triphosphate (ATP)ase 2a (SERCA2a) in myocardial tissues in vivo . (a) NE levels in myocardial tissue (n = 3 per group). (b) Representative Western blot images of stimulatory G protein (Gs), phosphorylated PKA (p-PKA), Cav1.2, p-PLB s16 and SERCA2a. (c–g) Quantification of Gs, p-PKA, Cav1.2 p-PLB s16 and SERCA2a protein expression levels (n = 3 per group). (h, i) Levels of adenylate cyclase (AC) and cyclic adenosine monophosphate (cAMP) in myocardial tissue (n = 3 per group). Data are mean ± SD. * p < 0.05, ** p < 0.01.

Article Snippet: After that, the film was transferred with 5% skim milk powder and then incubated with the following primary antibodies in the refrigerator overnight at 4°C: anti-β 1 (1:1000; Ab8226, Abcam, Cambridge, UK); anti-Gs (1:500; Ab235956, Abcam); anti-phosphorylated (p)-PKA (1:2500; Ab75991, Abcam); anti-Cav1.2 (1:500; ACC-003, Alomone Labs, Israel); anti-p-phospholamban (PLB; 1:10,000; Ab92697, Abcam); anti-sarcoplasmic reticulum (anti-SR) Ca 2+ -ATPase 2a (SERCA2a; 1:10,000; A010–23S, Badrilla, China); anti-cleaved caspase 3 (1:500; Ab32042, Abcam); and anti-β-actin (1:1000; Ab8226, Abcam).

Techniques: Expressing, In Vivo, Western Blot

Journal: Acupuncture in Medicine

Article Title: Electroacupuncture alleviates acute myocardial ischemic injury in mice by regulating the β 1 adrenergic receptor and post-receptor protein kinase A signaling pathway

doi: 10.1177/09645284241298716

Figure Lengend Snippet: Schematic representation of the cardiac β 1 adrenergic receptor (β 1 -AR) and post-receptor protein kinase A (PKA) signaling pathway regulated by electroacupuncture (EA). MI: myocardial ischemia; LAD: left anterior descending coronary artery; NE: norepinephrine; Gs: stimulatory G protein; AC: adenylate cyclase; cAMP: cyclic adenosine monophosphate; Cav1.2: L-type voltage-gated calcium channel α1 C; p-PLB s16 : serine phosphate 16-phospholamban; SERCA2a: sarcoplasmic reticulum Ca 2+ -adenosine triphosphate (ATP)ase 2a.

Article Snippet: After that, the film was transferred with 5% skim milk powder and then incubated with the following primary antibodies in the refrigerator overnight at 4°C: anti-β 1 (1:1000; Ab8226, Abcam, Cambridge, UK); anti-Gs (1:500; Ab235956, Abcam); anti-phosphorylated (p)-PKA (1:2500; Ab75991, Abcam); anti-Cav1.2 (1:500; ACC-003, Alomone Labs, Israel); anti-p-phospholamban (PLB; 1:10,000; Ab92697, Abcam); anti-sarcoplasmic reticulum (anti-SR) Ca 2+ -ATPase 2a (SERCA2a; 1:10,000; A010–23S, Badrilla, China); anti-cleaved caspase 3 (1:500; Ab32042, Abcam); and anti-β-actin (1:1000; Ab8226, Abcam).

Techniques: